6Q8U

Structure of the standard kink turn HmKt-7 variant A2bm6A bound with AfL7Ae protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-14
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9197
Spacegroup name	P 21 21 21
Unit cell lengths	57.360, 60.800, 119.990
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.415 - 1.990
R-factor	0.1906
Rwork	0.189
R-free	0.22720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4bw0
RMSD bond length	0.012
RMSD bond angle	1.345
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.415	2.020
High resolution limit [Å]	1.990	1.990
Rmerge	0.056	1.000
Rpim	0.025	0.440
Number of reflections	29389	1459
<I/σ(I)>	12.73	1.5
Completeness [%]	99.6	99.7
Redundancy	5.7	6
CC(1/2)	0.998	0.750

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	290	0.001 M Spermine tetrahydrochloride, 0.05 M MES pH6.5, 25 % v/v PEG 400