6Q8T

Cryo structure of HEWL at 81 kGy

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID13
Synchrotron site	ESRF
Beamline	ID13
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-09-16
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.9219
Spacegroup name	P 43 21 2
Unit cell lengths	77.200, 77.200, 37.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.300 - 1.740
R-factor	0.198716117342
Rwork	0.195
R-free	0.24198
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ydg
RMSD bond length	0.010
RMSD bond angle	1.108
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.770
High resolution limit [Å]	1.740	1.740
Number of reflections	13066	802
<I/σ(I)>	11.45	2.4
Completeness [%]	100.0	100
Redundancy	689	266

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	4	293	20 % NaCl 6 % PEG 6000 150 mM ammonium acetate pH 4.0