6Q8J
Nterminal domain of human SMU1 in complex with LSP641
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-25 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 72.193, 72.193, 183.164 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.720 - 1.800 |
| R-factor | 0.16359 |
| Rwork | 0.161 |
| R-free | 0.22007 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6q8f |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.457 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.000 | 1.880 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmeas | 0.051 | 0.762 |
| Number of reflections | 26383 | 3140 |
| <I/σ(I)> | 16.86 | 2.2 |
| Completeness [%] | 97.4 | 97.7 |
| Redundancy | 5.27 | 5.32 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 0.1 M Bis-Tris pH 6, 16-20 % PEG 10K, 0.2 M ammonium acetate |
