6Q8I
Nterminal domain of human SMU1 in complex with human REDmid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-02-14 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 127.983, 68.163, 145.296 |
Unit cell angles | 90.00, 109.40, 90.00 |
Refinement procedure
Resolution | 49.000 - 3.170 |
R-factor | 0.257 |
Rwork | 0.256 |
R-free | 0.27700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6q8f |
RMSD bond length | 0.008 |
RMSD bond angle | 0.980 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.000 | 3.250 |
High resolution limit [Å] | 3.170 | 3.170 |
Rmeas | 0.144 | 1.146 |
Number of reflections | 39441 | 2828 |
<I/σ(I)> | 6.9 | 1.14 |
Completeness [%] | 94.3 | 97.2 |
Redundancy | 2.4 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M Hepes pH 7.0-7.5, 8-10 % PEG 8K |