6Q7T
ERK2 mini-fragment binding
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-X |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-05-02 |
Detector | DECTRIS PILATUS 300K |
Wavelength(s) | 1.54178 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 48.685, 69.638, 59.796 |
Unit cell angles | 90.00, 109.06, 90.00 |
Refinement procedure
Resolution | 38.420 - 1.600 |
R-factor | 0.1832 |
Rwork | 0.181 |
R-free | 0.22672 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.010 |
RMSD bond angle | 1.411 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 56.520 | 1.620 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmeas | 0.047 | 1.229 |
Number of reflections | 32837 | 71 |
<I/σ(I)> | 11.2 | |
Completeness [%] | 65.6 | 8.3 |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 293 | 02M (NH4)2SO4 33% MPEG 2000 0.02M Mercaptoethanol 0.1M pH=7.2 HEPES/NaOH |