6Q7S
ERK2 mini-fragment binding
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-X |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-05-04 |
Detector | DECTRIS PILATUS 300K |
Wavelength(s) | 1.54178 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 48.628, 69.791, 60.169 |
Unit cell angles | 90.00, 108.81, 90.00 |
Refinement procedure
Resolution | 56.950 - 1.730 |
R-factor | 0.1865 |
Rwork | 0.184 |
R-free | 0.23988 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.010 |
RMSD bond angle | 1.443 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 56.950 | 1.750 |
High resolution limit [Å] | 1.730 | 1.730 |
Rmeas | 0.057 | 1.522 |
Number of reflections | 33133 | 887 |
<I/σ(I)> | 10.1 | |
Completeness [%] | 83.9 | 75.9 |
Redundancy | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | 02M (NH4)2SO4 33% MPEG 2000 0.02M Mercaptoethanol 0.1M pH=7.2 HEPES/NaOH |