6Q7K
ERK2 mini-fragment binding
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-X |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-05 |
| Detector | DECTRIS PILATUS 300K |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.643, 69.658, 60.563 |
| Unit cell angles | 90.00, 109.31, 90.00 |
Refinement procedure
| Resolution | 57.200 - 1.840 |
| R-factor | 0.21362 |
| Rwork | 0.211 |
| R-free | 0.26315 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.201 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.200 | 1.870 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Rmeas | 0.105 | 1.947 |
| Number of reflections | 33119 | 1474 |
| <I/σ(I)> | 6.1 | |
| Completeness [%] | 96.4 | 89.6 |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 293 | 0.2M (NH4)2SO4 33% MPEG 2000 0.02M Mercaptoethanol 0.1M pH=7.2 HEPES/NaOH |
