6Q78
The structure of GH26C from Muricauda sp. MAR_2010_75
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, DESY BEAMLINE P11 |
Synchrotron site | PETRA III, DESY |
Beamline | P11 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-09-25 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.0332 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 94.119, 60.358, 148.995 |
Unit cell angles | 90.00, 95.92, 90.00 |
Refinement procedure
Resolution | 48.839 - 1.500 |
R-factor | 0.165 |
Rwork | 0.164 |
R-free | 0.19060 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2vx6 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.066 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.400 | 1.520 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.117 | |
Rpim | 0.053 | 0.424 |
Number of reflections | 132761 | 6137 |
<I/σ(I)> | 9.5 | 2.2 |
Completeness [%] | 99.5 | 93.6 |
Redundancy | 6.7 | 6.4 |
CC(1/2) | 0.995 | 0.668 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.2 M Li2SO4, 0.1 M Bis-Tris:HCl pH 5.5, 24 % w/v PEG3350 |