6Q6Z

Structure of the plant immune signaling node EDS1 (enhanced disease susceptibility 1) in complex with nanobody ENB21

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.976254
Spacegroup name	P 3 2 1
Unit cell lengths	142.192, 142.192, 97.802
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	61.571 - 3.476
R-factor	0.2214
Rwork	0.218
R-free	0.26800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4nfu
RMSD bond length	0.003
RMSD bond angle	0.765
Data reduction software	XDS (XDS VERSION JAN 26, 2018, AUTOPROC VERSION JAN 26, 2018)
Data scaling software	autoPROC (autoPROC (Version 1.0.5), AIMLESS (version 0.7.3), CCP4 7.0.065, STARANISO)
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	76.586	3.646
High resolution limit [Å]	3.473	3.473
Rmerge	0.182
Rmeas	0.187
Rpim	0.043
Number of reflections	12634	634
<I/σ(I)>	12.1
Completeness [%]	90.8	57.1
Redundancy	19.5	20.1
CC(1/2)	0.993	0.354

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277.15	CRYSTALLIZATION CONDITIONS: RESERVOIR COMPOSITION (CONDITION A11 of MORPHEUS SCREEN): 20.0 % (V/V) GLYCEROL, 10.0 % (W/V) PEG 4000, 0.03 M MAGNESIUM CHLORIDE, 0.03 M CALCIUM CHLORIDE, 0.0609 M TRIS-BASE, 0.0391 M BICINE, PH 8.5; PROTEIN STOCK SOLUTION: 4.1 MG/ML PROTEIN, 50 MM SODIUM CHLORIDE, 1 % (V/V) GLYCEROL, 1 MM DTT, 50 MM HEPES, PH 8.0; DROP COMPOSITION: 150 NL PROTEIN STOCK SOLUTION PLUS 150 NL RESERVOIR SOLUTION; CRYO CONDITIONS: THE CRYSTALS WERE FLASH FROZEN DIRECTLY FROM THE EQUILIBRATED CRYSTALLIZATION DROPS.