6Q6B

Structure of the copper storage protein, Ccsp, from Streptomyces lividans loaded with 10 copper equivalents

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.33
Spacegroup name	P 61 2 2
Unit cell lengths	93.398, 93.398, 216.015
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	216.010 - 1.900
R-factor	0.2195
Rwork	0.218
R-free	0.25500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ei0
RMSD bond length	0.015
RMSD bond angle	1.639
Data reduction software	TRUNCATE
Data scaling software	Aimless (0.6.2)
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	216.010	75.750	1.940
High resolution limit [Å]	1.900	9.110	1.900
Rmerge	0.102	0.069	1.813
Rmeas	0.108	0.073	1.926
Rpim	0.035	0.022	0.636
Number of reflections	44544	474	2831
<I/σ(I)>	12.8
Completeness [%]	99.7	95.6	100
Redundancy	9.1	8.8	9.2
CC(1/2)	0.998	0.998	0.464

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1.5 M Ammonium Sulfate, 0.1M MES pH6.0