6Q61

Pore-modulating toxins exploit inherent slow inactivation to block K+ channels

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2011-11-03
Detector	ADSC QUANTUM 4
Wavelength(s)	0.98
Spacegroup name	P 63
Unit cell lengths	51.031, 51.031, 42.569
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.620 - 1.300
R-factor	0.14
Rwork	0.139
R-free	0.16741
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1y62
RMSD bond length	0.018
RMSD bond angle	1.973
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.620	1.347
High resolution limit [Å]	1.300	1.300
Rpim	0.013	0.293
Number of reflections	15585	1554
<I/σ(I)>	24.99
Completeness [%]	99.8	99.61
Redundancy	10.8	10.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	298	0.2 M Ammonium sulfate, 0.1 M Sodium acetate trihydrate pH 4.6 and 25% w/v PEG 4,000