6Q61
Pore-modulating toxins exploit inherent slow inactivation to block K+ channels
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-11-03 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.98 |
Spacegroup name | P 63 |
Unit cell lengths | 51.031, 51.031, 42.569 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.620 - 1.300 |
R-factor | 0.14 |
Rwork | 0.139 |
R-free | 0.16741 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1y62 |
RMSD bond length | 0.018 |
RMSD bond angle | 1.973 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.620 | 1.347 |
High resolution limit [Å] | 1.300 | 1.300 |
Rpim | 0.013 | 0.293 |
Number of reflections | 15585 | 1554 |
<I/σ(I)> | 24.99 | |
Completeness [%] | 99.8 | 99.61 |
Redundancy | 10.8 | 10.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 0.2 M Ammonium sulfate, 0.1 M Sodium acetate trihydrate pH 4.6 and 25% w/v PEG 4,000 |