6Q5T
Crystal structure of Mycolicibacterium hassiacum glucosylglycerate hydrolase (MhGgH) - apo form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-11-27 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.96863 |
Spacegroup name | P 62 2 2 |
Unit cell lengths | 167.008, 167.008, 243.266 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.860 - 2.540 |
R-factor | 0.1682 |
Rwork | 0.166 |
R-free | 0.20380 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ohz |
RMSD bond length | 0.009 |
RMSD bond angle | 1.058 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.860 | 2.630 |
High resolution limit [Å] | 2.540 | 2.540 |
Rmerge | 0.109 | 1.165 |
Rmeas | 0.117 | 1.254 |
Rpim | 0.043 | 0.459 |
Number of reflections | 66272 | |
<I/σ(I)> | 11.9 | 1.8 |
Completeness [%] | 99.8 | 99.7 |
Redundancy | 7.2 | 7.3 |
CC(1/2) | 0.998 | 0.673 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | ADA pH 6.5, ammonium sulfate |