6Q5G
The ABC transporter associated binding protein from B. animalis subsp. lactis Bl-04 without ligand. SeMet variant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-18 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97901 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.935, 69.364, 97.297 |
| Unit cell angles | 90.00, 104.43, 90.00 |
Refinement procedure
| Resolution | 47.113 - 2.000 |
| R-factor | 0.1778 |
| Rwork | 0.177 |
| R-free | 0.20040 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.551 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AutoSol (1.11.1) |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.000 | 2.071 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.095 | |
| Number of reflections | 54411 | |
| <I/σ(I)> | 13.35 | |
| Completeness [%] | 98.9 | |
| Redundancy | 6.6 | |
| CC(1/2) | 0.996 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 297 | 12.5% PEG 1000, 12.5% PEG 3350, 12.5% MDP, 30 mM each of di-,tri-,tetra- and pentaethyleneglycol with 0.1M MES/imidazole at pH6.5 |
