6Q58
Copper loading to a cytosolic copper storage protein from Streptomyces lividans (five coppers)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-11-11 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1.33 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 93.051, 93.051, 212.340 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 212.340 - 1.500 |
R-factor | 0.1955 |
Rwork | 0.195 |
R-free | 0.21350 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6ei0 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.472 |
Data reduction software | DIALS |
Data scaling software | Aimless (0.6.2) |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 212.340 | 75.340 | 1.530 |
High resolution limit [Å] | 1.500 | 8.220 | 1.500 |
Rmerge | 0.057 | 0.054 | 0.659 |
Rmeas | 0.060 | 0.058 | 0.801 |
Rpim | 0.020 | 0.018 | 0.447 |
Number of reflections | 85587 | 628 | 3492 |
<I/σ(I)> | 19.5 | ||
Completeness [%] | 98.0 | 96.2 | 82.5 |
Redundancy | 8 | 8.7 | 2.9 |
CC(1/2) | 0.999 | 0.994 | 0.448 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1.5 M Ammonium Sulfate 0.1M MES pH 6.0 |