6Q4X

Structure of MPT-2, a GDP-Man-dependent mannosyltransferase from Leishmania mexicana

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	55.567, 98.607, 107.402
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	72.740 - 1.550
R-factor	0.1436
Rwork	0.140
R-free	0.21091
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.575
Data reduction software	DIALS (1.8.2-g5331de77-release)
Data scaling software	Aimless (0.7.3)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	72.740	1.580
High resolution limit [Å]	1.550	1.550
Rmerge	0.103	0.756
Rmeas	0.113	0.859
Rpim	0.045	0.395
Number of reflections	84940	3419
<I/σ(I)>	7.1
Completeness [%]	98.3	81.1
Redundancy	5.9	3.9
CC(1/2)	0.997	0.430

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		292	200 mM trisodium citrate, 18-22% w/v PEG 3350