6Q40

A secreted LysM effector of the wheat pathogen Zymoseptoria tritici protects the fungal hyphae against chitinase hydrolysis through ligand-dependent polymerisation of LysM homodimers

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-01-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.91841
Spacegroup name	P 61 2 2
Unit cell lengths	119.390, 119.390, 157.670
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.452 - 2.412
R-factor	0.1815
Rwork	0.180
R-free	0.22030
Structure solution method	SAD
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.452	2.509
High resolution limit [Å]	2.412	2.412
Number of reflections	26300	2827
<I/σ(I)>	18
Completeness [%]	92.1	100
Redundancy	10.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	Protein stock: 20 mM HEPES pH 7.0, and 50 mM NaCl Initial crystals: Reservoir 47.5% dioxan Seeds stock for micro-seeding: 45% dioxan Best conditions: By micro-seeding techniques using 0.1 M sodium citrate pH 5.6, 5%-20% PEG4000 and 5% isopropanol as the reservoir solution. Crystal cryo and soaking buffer: 0.2 M sodium acetate pH 4.6 with 20% ethylene glycol.