6Q3Q

Arabidopsis OM64 TPR domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	293
Detector technology	PIXEL
Collection date	2015-11-24
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.541790
Spacegroup name	P 63
Unit cell lengths	148.630, 148.630, 47.290
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.000
R-factor	0.1367
Rwork	0.135
R-free	0.16820
RMSD bond length	0.013
RMSD bond angle	1.570
Data reduction software	ADDREF
Data scaling software	Aimless (3.3.22)
Phasing software	ACORN
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000	20.000	2.110
High resolution limit [Å]	2.000	6.320	2.000
Rmerge	0.154	0.082	0.660
Rmeas	0.162	0.086	0.698
Rpim	0.051	0.027	0.225
Total number of observations	407295
Number of reflections	40693	1331	5897
<I/σ(I)>	11.7
Completeness [%]	99.9	96.9	100
Redundancy	10	10.2	9.6
CC(1/2)	0.994	0.996	0.815

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277.15	0.10 M Bis-Tris pH 6.20, 0.10 M Na-Chloride, 1.10 M (NH4)2-Sulfate, 0.05 M Mg-Chloride, 0.1 M Na-Acetate, pH 4.60, 2 M (NH4)2-Sulfate