6Q2K
The structure of the Streptococcus gordonii surface protein SspB in complex with TEV peptide provides clues to the adherence of oral streptococcal adherence to salivary agglutinin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-03-10 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 121.505, 121.505, 50.745 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.470 - 2.000 |
| R-factor | 0.1955 |
| Rwork | 0.194 |
| R-free | 0.23550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wd6 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.111 |
| Data reduction software | HKL-2000 (2.3.8) |
| Data scaling software | HKL-2000 (2.3.8) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.118 | 0.445 |
| Rmeas | 0.122 | 0.527 |
| Rpim | 0.027 | 0.275 |
| Number of reflections | 25664 | 1071 |
| <I/σ(I)> | 9.9 | 2.4 |
| Completeness [%] | 97.7 | 82.8 |
| Redundancy | 15.5 | 3.1 |
| CC(1/2) | 0.839 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 3M Sodium acetate trihydrate, 0.1M Hepes, pH 7.0 |
