6Q2F

Structure of Rhamnosidase from Novosphingobium sp. PP1Y

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-14
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.979
Spacegroup name	P 2 21 21
Unit cell lengths	80.809, 114.570, 158.797
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.699 - 2.200
R-factor	0.163541015328
Rwork	0.163
R-free	0.17705
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.027
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.700	2.250
High resolution limit [Å]	2.200	2.200
Number of reflections	144262	29836
<I/σ(I)>	14.2	3.7
Completeness [%]	99.9
Redundancy	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1 M MES, pH 6.8, 6-7% Tascimate pH 5.0, 2-10 mM Tris, pH 7.5, 10 % w/w PEG 20K, 6-10% glycerol, and 0.01% NaN3