6Q26
N-Acetylmannosamine kinase from Staphylococcus aureus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2016-11-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9724 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 116.647, 135.149, 175.330 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.736 - 2.328 |
| R-factor | 0.2017 |
| Rwork | 0.197 |
| R-free | 0.24000 |
| Structure solution method | SAD |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | SHELXDE |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.736 | 2.412 |
| High resolution limit [Å] | 2.328 | 2.328 |
| Number of reflections | 867330 | 5864 |
| <I/σ(I)> | 20 | |
| Completeness [%] | 99.9 | |
| Redundancy | 14.6 | |
| CC(1/2) | 0.994 | 0.671 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | Molecular Dimensions JCSG G6: 0.2 M sodium malonate dibasic monohydrate 20% w/v PEG 3350 |
