6Q23
Crystal structure of human 1G01 Fab in complex with influenza virus neuraminidase from A/California/04/2009 (H1N1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-02-16 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.03309 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 106.594, 234.985, 106.275 |
Unit cell angles | 90.00, 106.40, 90.00 |
Refinement procedure
Resolution | 43.300 - 3.270 |
R-factor | 0.19841 |
Rwork | 0.196 |
R-free | 0.24078 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3nss 5itb 4fql |
RMSD bond length | 0.008 |
RMSD bond angle | 1.384 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.300 | 3.380 |
High resolution limit [Å] | 3.270 | 3.270 |
Rpim | 0.120 | 0.430 |
Number of reflections | 70214 | 3688 |
<I/σ(I)> | 4.5 | 1.1 |
Completeness [%] | 91.9 | 73.1 |
Redundancy | 2.7 | 2.2 |
CC(1/2) | 0.952 | 0.876 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1 M Tris, pH 8.5, 10% (v/v) glycerol, 20% (w/v) PEG300, 5% (w/v) PEG8000 |