6Q1K

Inferred intermediate I-7 of the human antibody lineage 652

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	80
Detector technology	CCD
Collection date	2014-12-18
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	47.160, 69.990, 65.230
Unit cell angles	90.00, 108.61, 90.00

Refinement procedure

Resolution	46.334 - 2.200
R-factor	0.1934
Rwork	0.191
R-free	0.23940
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.334	2.279
High resolution limit [Å]	2.200	2.200
Rmerge	0.095
Number of reflections	20469	2025
<I/σ(I)>	12
Completeness [%]	99.5
Redundancy	3.7
CC(1/2)	0.996	0.827

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	291.15	200mM sodium acetate, 30% (w/v) PEG 400, 100mM Tris-HCl pH 8.5