6Q1J
Antibody H2227 from the human antibody lineage 652
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2014-10-17 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9793 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 57.300, 69.380, 142.820 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.691 - 2.703 |
R-factor | 0.2263 |
Rwork | 0.225 |
R-free | 0.24560 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.691 | 44.180 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.139 | 0.970 |
Number of reflections | 15536 | 1530 |
<I/σ(I)> | 13.65 | |
Completeness [%] | 95.8 | 95.98 |
Redundancy | 5.6 | |
CC(1/2) | 0.998 | 0.703 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4 | 291.15 | 100mM ammonium sulfate, 40% (v/v) PEG 400, 100mM sodium citrate pH 4.0 |