6Q1I
GH5-4 broad specificity endoglucanase from Clostrdium longisporum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-10-13 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.978560 |
Spacegroup name | P 1 |
Unit cell lengths | 45.350, 53.480, 68.060 |
Unit cell angles | 104.05, 96.89, 87.06 |
Refinement procedure
Resolution | 25.210 - 1.350 |
R-factor | 0.1119 |
Rwork | 0.112 |
R-free | 0.13170 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ndy |
RMSD bond length | 0.005 |
RMSD bond angle | 0.850 |
Data reduction software | XDS (20190315) |
Data scaling software | XSCALE (20190315) |
Phasing software | PHASER |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.210 | 1.398 |
High resolution limit [Å] | 1.350 | 1.350 |
Rmerge | 0.040 | 0.108 |
Rmeas | 0.047 | 0.130 |
Rpim | 0.024 | 0.071 |
Number of reflections | 128382 | 12528 |
<I/σ(I)> | 20.86 | 8.28 |
Completeness [%] | 94.8 | |
Redundancy | 3.8 | |
CC(1/2) | 0.998 | 0.988 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | Crystals were set with a Mosquito into SD2 plates. The condition that provided the crystals for this data set came from Morpheus Screen D9: 10% Alcohols, 50% Tris/Bicine H 8.5, and 15% P550MME_P20K |