6Q1G
Human antibody H1244 of the human antibody lineage 652
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2017-09-20 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 67.140, 67.470, 95.690 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.612 - 1.750 |
R-factor | 0.1809 |
Rwork | 0.180 |
R-free | 0.20640 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.612 | 1.813 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.068 | 1.225 |
Number of reflections | 44531 | 4380 |
<I/σ(I)> | 20.5 | |
Completeness [%] | 99.9 | 99.91 |
Redundancy | 8 | |
CC(1/2) | 0.999 | 0.660 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | 100mM Potassium thiocyanate, 30% (w/v) PEG 2000 MME |