6Q1E
Inferred precursor (UCA) of the human antibody lineage 652
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2014-12-18 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 45.440, 69.670, 74.440 |
Unit cell angles | 90.00, 100.08, 90.00 |
Refinement procedure
Resolution | 44.738 - 2.500 |
R-factor | 0.2245 |
Rwork | 0.223 |
R-free | 0.25740 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.738 | 2.589 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.132 | 1.031 |
Number of reflections | 15923 | 1576 |
<I/σ(I)> | 10.85 | |
Completeness [%] | 99.7 | |
Redundancy | 3.7 | |
CC(1/2) | 0.993 | 0.579 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 291.15 | 5% (v/v) PEG 400, 1.4M sodium citrate pH 5.0 |