6Q1C

Apo YfeA extracted from the E. coli periplasm

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-07
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.28242
Spacegroup name	P 21 21 21
Unit cell lengths	40.017, 52.426, 113.263
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.472 - 1.760
R-factor	0.1941
Rwork	0.190
R-free	0.23850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5uxs
Data reduction software	HKL-2000 (v712)
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.790
High resolution limit [Å]	1.760	1.760
Number of reflections	43178	2960
<I/σ(I)>	35.4
Completeness [%]	98.5
Redundancy	3.5
CC(1/2)	0.923	0.754

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.3	293.15	20 mM Bis-Tris, pH 6.3, 50 mM sodium chloride, 0.05% w/v sodium azide, 30% w/v PEG4000