6Q1A
Inferred intermediate mutant (I-6V) of the human antibody lineage 652
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2016-05-26 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9999 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 70.021, 71.780, 90.604 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.859 - 2.001 |
R-factor | 0.2044 |
Rwork | 0.203 |
R-free | 0.22470 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.859 | 2.073 |
High resolution limit [Å] | 2.001 | 2.001 |
Rmerge | 0.067 | 0.822 |
Number of reflections | 31446 | 3073 |
<I/σ(I)> | 25.32 | |
Completeness [%] | 99.9 | 99.71 |
Redundancy | 7.2 | |
CC(1/2) | 0.999 | 0.795 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291.15 | 200 mM zinc acetate, 10% (w/v) PEG 8000 100 mM MES pH 6.0 |