6Q19
Inferred intermediate (I-6) of the human antibody lineage 652
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2016-05-26 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9998 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 47.770, 70.030, 65.650 |
Unit cell angles | 90.00, 108.21, 90.00 |
Refinement procedure
Resolution | 46.573 - 1.580 |
R-factor | 0.1652 |
Rwork | 0.164 |
R-free | 0.19100 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.573 | 1.637 |
High resolution limit [Å] | 1.580 | 1.580 |
Rmerge | 0.061 | 0.671 |
Number of reflections | 55518 | 4982 |
<I/σ(I)> | 14.25 | |
Completeness [%] | 98.5 | 89.19 |
Redundancy | 3.5 | |
CC(1/2) | 0.999 | 0.517 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291.15 | 1M sodium chloride, 30% (w/v) PEG 8000, 100 mM HEPES pH7.0 |