6Q0I

Inferred intermediate I-7 (I-7-6) of the human antibody lineage 652 in complex with influenza hemagglutinin head domain of A/Beijing/262/95(H1N1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	80
Detector technology	CCD
Collection date	2018-06-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	51.950, 70.410, 203.740
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.881 - 2.702
R-factor	0.2156
Rwork	0.213
R-free	0.25540
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.881	2.799
High resolution limit [Å]	2.702	2.702
Rmerge	0.072	1.038
Number of reflections	21279	18093
<I/σ(I)>	24.15
Completeness [%]	99.8	99.23
Redundancy	8.7
CC(1/2)	0.999	0.896

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	291.15	100mM lithium sulfate, 30% (w/v) PEG 6000, 100mM CHES