6Q0H
Inferred intermediate I-7 (I-7-0) of the human antibody lineage 652 in complex with influenza hemagglutinin head domain of A/Beijing/262/95(H1N1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2018-06-20 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 52.810, 70.580, 200.940 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.585 - 2.750 |
R-factor | 0.2426 |
Rwork | 0.241 |
R-free | 0.26920 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.585 | 2.848 |
High resolution limit [Å] | 2.750 | 2.750 |
Rmerge | 0.069 | 0.951 |
Number of reflections | 20295 | 1980 |
<I/σ(I)> | 23.15 | 2.2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 8.6 | 9 |
CC(1/2) | 0.999 | 0.780 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291.15 | 5% (v/v) PEG 400, 1.1M sodium citrate |