6Q0E

Inferred precursor (UCA) of the human antibody lineage 652 in complex with influenza hemagglutinin head domain of A/Beijing/262/95(H1N1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	291.15
Detector technology	CCD
Collection date	2017-05-26
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	52.030, 69.780, 200.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.292 - 2.150
R-factor	0.2312
Rwork	0.230
R-free	0.24850
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.292	2.227
High resolution limit [Å]	2.150	2.150
Rmerge	0.141
Number of reflections	40694	4005
<I/σ(I)>	13.21
Completeness [%]	99.5	99.78
Redundancy	7.05
CC(1/2)	0.997	0.801

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291.15	100 mM sodium chloride, 30% (v/v) PEG 400, 100 mM MES