6Q00
TDP2 UBA Domain Bound to Ubiquitin at 0.85 Angstroms Resolution, Crystal Form 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-04-25 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 0.8266 |
Spacegroup name | P 41 |
Unit cell lengths | 48.742, 48.742, 50.421 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.453 - 0.850 |
R-factor | 0.0958 |
Rwork | 0.095 |
R-free | 0.11180 |
Structure solution method | SAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.469 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 0.860 |
High resolution limit [Å] | 0.850 | 2.310 | 0.850 |
Rmerge | 0.048 | 0.042 | |
Rmeas | 0.049 | 0.043 | |
Rpim | 0.009 | 0.008 | 0.443 |
Number of reflections | 202939 | 5282 | 5067 |
<I/σ(I)> | 15.3 | ||
Completeness [%] | 99.6 | 100 | 97.8 |
Redundancy | 16.1 | 34.3 | 8.6 |
CC(1/2) | 1.000 | 0.574 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 278 | 10 mM Tris, 200 mM Potassium Formate, 14% (w/v) PEG 3350 |