Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-07 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.95373 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 87.620, 89.960, 130.529 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.240 - 2.000 |
| R-factor | 0.1877 |
| Rwork | 0.186 |
| R-free | 0.22290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3u5t |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.781 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.240 | 2.040 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.099 | 0.346 |
| Rmeas | 0.116 | 0.405 |
| Rpim | 0.060 | 0.207 |
| Number of reflections | 70331 | 4491 |
| <I/σ(I)> | 7.5 | 3.6 |
| Completeness [%] | 99.9 | |
| Redundancy | 3.6 | |
| CC(1/2) | 0.843 | 0.772 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 296.15 | 0.1 M HEPES, pH 7.5, 0.8 M potassium sodium tartrate |
