6PZG

Crystal structure of human NA-80 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-16
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97946
Spacegroup name	P 32 2 1
Unit cell lengths	102.770, 102.770, 80.359
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.500 - 1.590
R-factor	0.1702
Rwork	0.168
R-free	0.20360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4kmt 5aze
RMSD bond length	0.010
RMSD bond angle	1.061
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.500	1.620
High resolution limit [Å]	1.590	1.590
Rpim	0.020	0.350
Number of reflections	66498	3234
<I/σ(I)>	58	1.3
Completeness [%]	99.9	98.3
Redundancy	18.1
CC(1/2)	0.999	0.630

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	293	0.1 M sodium citrate, pH 5.6, 40% PEG600