6PZG
Crystal structure of human NA-80 Fab
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-11-16 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97946 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 102.770, 102.770, 80.359 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.500 - 1.590 |
R-factor | 0.1702 |
Rwork | 0.168 |
R-free | 0.20360 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4kmt 5aze |
RMSD bond length | 0.010 |
RMSD bond angle | 1.061 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.500 | 1.620 |
High resolution limit [Å] | 1.590 | 1.590 |
Rpim | 0.020 | 0.350 |
Number of reflections | 66498 | 3234 |
<I/σ(I)> | 58 | 1.3 |
Completeness [%] | 99.9 | 98.3 |
Redundancy | 18.1 | |
CC(1/2) | 0.999 | 0.630 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 0.1 M sodium citrate, pH 5.6, 40% PEG600 |