6PYK
P. mirabilis hemolysin A mutant - F80L
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-20 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 51.550, 34.011, 59.480 |
| Unit cell angles | 90.00, 101.02, 90.00 |
Refinement procedure
| Resolution | 34.011 - 1.350 |
| R-factor | 0.1344 |
| Rwork | 0.133 |
| R-free | 0.16150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fy3 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.15_3459) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.011 | 1.390 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.085 | 0.804 |
| Rpim | 0.034 | 0.331 |
| Number of reflections | 44578 | 4273 |
| <I/σ(I)> | 15.9 | 2.1 |
| Completeness [%] | 99.3 | 95.1 |
| Redundancy | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | PEG 4000 |
