6PYK
P. mirabilis hemolysin A mutant - F80L
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-06-20 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.97872 |
Spacegroup name | P 1 2 1 |
Unit cell lengths | 51.550, 34.011, 59.480 |
Unit cell angles | 90.00, 101.02, 90.00 |
Refinement procedure
Resolution | 34.011 - 1.350 |
R-factor | 0.1344 |
Rwork | 0.133 |
R-free | 0.16150 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3fy3 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.15_3459) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.011 | 1.390 |
High resolution limit [Å] | 1.350 | 1.350 |
Rmerge | 0.085 | 0.804 |
Rpim | 0.034 | 0.331 |
Number of reflections | 44578 | 4273 |
<I/σ(I)> | 15.9 | 2.1 |
Completeness [%] | 99.3 | 95.1 |
Redundancy | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | PEG 4000 |