6PXZ

Crystal Structure of mouse Serum Amyloid A3 (SAA3) in the trimeric form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-12-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	52.317, 74.882, 89.940
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.840 - 1.700
R-factor	0.219
Rwork	0.217
R-free	0.25190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4q5g
RMSD bond length	0.007
RMSD bond angle	0.893
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.8_1069)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.730
High resolution limit [Å]	1.700	4.610	1.700
Rmerge	0.071	0.036	1.461
Rmeas	0.075	0.039	1.561
Rpim	0.026	0.014	0.544
Total number of observations	333363
Number of reflections	39758	2145	1945
<I/σ(I)>	7.7
Completeness [%]	99.9	98.8	100
Redundancy	8.4	7.8	8.1
CC(1/2)		0.998	0.569

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	0.2 M trisodium citrate, 0.1 M HEPES, pH 7.5, 6% PEG6000, 10% isopropanol