6PXE
Crystal structure of the complex between periplasmic domains of antiholin RI and holin T from T4 phage, in P21
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-11-12 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 78.590, 82.560, 81.433 |
| Unit cell angles | 90.00, 102.16, 90.00 |
Refinement procedure
| Resolution | 39.803 - 2.300 |
| R-factor | 0.2347 |
| Rwork | 0.232 |
| R-free | 0.29280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6psk |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.033 |
| Data reduction software | PROTEUM PLUS |
| Data scaling software | SAINT |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.800 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.151 | 0.787 |
| Number of reflections | 44230 | 2168 |
| <I/σ(I)> | 7.57 | |
| Completeness [%] | 97.4 | |
| Redundancy | 5.07 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 3.5 | 289 | 25% PEG 3,350 with 0.1M citric acid pH 3.5 |
