6PX4
Crystal structure of the complex between periplasmic domains of antiholin RI and holin T from T4 phage, in H32
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 120 |
Detector technology | CCD |
Collection date | 2016-04-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.987 |
Spacegroup name | H 3 2 |
Unit cell lengths | 116.546, 116.546, 223.384 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 33.644 - 1.650 |
R-factor | 0.1951 |
Rwork | 0.194 |
R-free | 0.22520 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.875 |
Data reduction software | SAINT |
Data scaling software | SAINT |
Phasing software | MOLREP |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 99.000 | 1.750 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.065 | |
Number of reflections | 68307 | 9569 |
<I/σ(I)> | 16.45 | |
Completeness [%] | 97.2 | 85.1 |
Redundancy | 7.29 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 0.8 M Na/K Tartrate |