6PWJ
Vibrio cholerae LapD S helix-GGDEF-EAL (apo)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-03 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.6309 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 129.830, 129.830, 114.441 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 12.690 - 2.700 |
| R-factor | 0.2017 |
| Rwork | 0.199 |
| R-free | 0.24270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.366 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless (0.5.8) |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 19.534 | 19.530 | 2.830 |
| High resolution limit [Å] | 2.700 | 8.950 | 2.700 |
| Rmerge | 0.095 | 0.068 | 0.414 |
| Rmeas | 0.102 | 0.074 | 0.445 |
| Rpim | 0.037 | 0.028 | 0.159 |
| Total number of observations | 4347 | 29666 | |
| Number of reflections | 29068 | 760 | 3868 |
| <I/σ(I)> | 14.6 | 22.9 | 4.7 |
| Completeness [%] | 94.6 | 83.9 | 95.7 |
| Redundancy | 7.3 | 5.7 | 7.7 |
| CC(1/2) | 0.996 | 0.991 | 0.922 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 2.5 M sodium acetate trihydrate, pH 7.0 15% glycerol |
