6PWB

Rigid body fitting of flagellin FlaB, and flagellar coiling proteins, FcpA and FcpB, into a 10 Angstrom structure of the asymmetric flagellar filament purified from Leptospira biflexa Patoc WT cells resolved via subtomogram averaging

This is a non-PDB format compatible entry.

EM specimen

Vitrification	Yes
Instrument	FEI VITROBOT MARK III

EM Data Collection

Microscope	FEI POLARA 300
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	300
Cs (mm)	2
Detector	GATAN K2 SUMMIT (4k x 4k)

3D Reconstruction

Method	SUBTOMOGRAM AVERAGING
Resolution (Å)	9.83
Software	Rosetta,Situs,UCSF Chimera,RELION,emClarity,PEET,SerialEM,IMOD
Symmetry	C1
CTF correction	PHASE FLIPPING AND AMPLITUDE CORRECTION