6PW7

X-ray crystal structure of C. elegans STIM EF-SAM domain

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-10
Detector	RAYONIX MX300HE
Wavelength(s)	0.97873, 0.97903, 0.9746, 0.98321
Spacegroup name	C 2 2 2
Unit cell lengths	111.543, 111.580, 70.704
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.800 - 1.890
R-factor	0.1897
Rwork	0.187
R-free	0.23550
Structure solution method	MAD
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.850
High resolution limit [Å]	1.820	1.820
Number of reflections	39514	1867
<I/σ(I)>	12.1
Completeness [%]	98.7
Redundancy	14.5
CC(1/2)	0.998	0.183

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	277	18% v/v PEG 3,350, 8% v/v tacsimate, 10mM calcium chloride