6PVV
RNase A in complex with p5A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | BRUKER IMUS MICROFOCUS |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-04-18 |
| Detector | Bruker PHOTON III |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 99.790, 32.250, 71.950 |
| Unit cell angles | 90.00, 90.30, 90.00 |
Refinement procedure
| Resolution | 29.250 - 1.650 |
| R-factor | 0.1936 |
| Rwork | 0.192 |
| R-free | 0.22250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1afu |
| Data reduction software | SAINT |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.250 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.086 | 0.503 |
| Rmeas | 0.090 | 0.597 |
| Rpim | 0.023 | 0.313 |
| Number of reflections | 27900 | 2635 |
| <I/σ(I)> | 18.2 | 2 |
| Completeness [%] | 99.5 | 95.85 |
| Redundancy | 12.4 | 3.06 |
| CC(1/2) | 0.999 | 0.623 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 20 mM sodium citrate, 20% PEG 4000 |
