6PVU
RNase A in complex with hexametaphosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | BRUKER IMUS MICROFOCUS |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-15 |
| Detector | Bruker PHOTON III |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 100.350, 32.640, 72.610 |
| Unit cell angles | 90.00, 90.65, 90.00 |
Refinement procedure
| Resolution | 29.570 - 1.490 |
| R-factor | 0.2033 |
| Rwork | 0.202 |
| R-free | 0.23590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1afu |
| Data reduction software | SAINT |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.570 | 1.550 |
| High resolution limit [Å] | 1.490 | 1.490 |
| Rmerge | 0.047 | 0.410 |
| Rmeas | 0.052 | 0.466 |
| Rpim | 0.022 | 0.217 |
| Number of reflections | 38224 | 3454 |
| <I/σ(I)> | 20.5 | 3.2 |
| Completeness [%] | 99.0 | 89.8 |
| Redundancy | 5.4 | 4.3 |
| CC(1/2) | 0.999 | 0.857 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 20 mM sodium citrate, 20% PEG 4000 |
