6PV5
Structure of CpGH84B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-02-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.00000 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 171.389, 171.389, 128.797 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.240 - 2.180 |
| R-factor | 0.1908 |
| Rwork | 0.188 |
| R-free | 0.23680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v5c |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.296 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.240 | 2.300 |
| High resolution limit [Å] | 2.180 | 2.180 |
| Rmerge | 0.079 | 0.491 |
| Rpim | 0.027 | 0.168 |
| Number of reflections | 37842 | 5487 |
| <I/σ(I)> | 18.2 | 5.8 |
| Completeness [%] | 100.0 | 99.8 |
| Redundancy | 9.7 | 9.4 |
| CC(1/2) | 0.998 | 0.943 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 0.1 M Tris HCl and 2.0 M ammonium sulphate |
