6PUN
Crystal structure of a ternary complex of FBF-2 with LST-1 (site B) and compact FBE RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-11-01 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 |
Unit cell lengths | 42.751, 74.380, 81.547 |
Unit cell angles | 107.17, 104.40, 101.76 |
Refinement procedure
Resolution | 42.691 - 2.099 |
R-factor | 0.2001 |
Rwork | 0.198 |
R-free | 0.24040 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3v74 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.140 |
High resolution limit [Å] | 2.099 | 5.700 | 2.100 |
Rmerge | 0.108 | 0.092 | 0.749 |
Rmeas | 0.118 | 0.099 | 0.813 |
Rpim | 0.045 | 0.038 | 0.312 |
Number of reflections | 50128 | 2562 | 2514 |
<I/σ(I)> | 9 | ||
Completeness [%] | 96.9 | 99 | 97.1 |
Redundancy | 6.6 | 7 | 6.5 |
CC(1/2) | 0.990 | 0.865 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 17-20% w/v PEG3350, 0.2 M magnesium chloride, 0.1 M MES |