6PU1

Cysteine stabilized hexameric HIV-1 CA in complex with SEC24C peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97741
Spacegroup name	P 6
Unit cell lengths	92.580, 92.580, 58.019
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.000 - 2.280
R-factor	0.2338
Rwork	0.232
R-free	0.27240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4u0a
RMSD bond length	0.002
RMSD bond angle	0.469
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.16_3546)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.000	2.360
High resolution limit [Å]	2.280	2.280
Rmerge	0.124
Number of reflections	13073	1264
<I/σ(I)>	11.8
Completeness [%]	100.0
Redundancy	10.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277.15	12% PEG3350, 24% glycerol, 0.35 M NaI, 50 mM sodium cacodylate pH 6.5