6PTT
Soluble model of Arabidopsis thaliana CuA (Tt3LAt)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-13 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.3679 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.335, 73.734, 79.135 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.070 - 1.840 |
R-factor | 0.1719 |
Rwork | 0.170 |
R-free | 0.21370 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2cua |
RMSD bond length | 0.020 |
RMSD bond angle | 1.193 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.14rc3_3199) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.070 | 1.880 |
High resolution limit [Å] | 1.840 | 1.840 |
Rmerge | 0.078 | 0.438 |
Rmeas | 0.089 | 0.508 |
Rpim | 0.043 | 0.254 |
Number of reflections | 26102 | 1572 |
<I/σ(I)> | 16.1 | 3 |
Completeness [%] | 98.2 | 99.5 |
Redundancy | 4.1 | 4.1 |
CC(1/2) | 0.990 | 0.896 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 291 | 100 mM Hepes, 1.5 M LiSO4, pH 7.5 |