6PTQ

Dark, Room Temperature, PCM Myxobacterial Phytochrome, P2, Wild Type

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	FREE ELECTRON LASER
Source details	SACLA BEAMLINE BL3
Synchrotron site	SACLA
Beamline	BL3
Temperature [K]	290
Detector technology	CCD
Collection date	2019-04-22
Detector	MPCCD
Wavelength(s)	1.38
Spacegroup name	P 1 21 1
Unit cell lengths	83.690, 83.400, 86.870
Unit cell angles	90.00, 107.63, 90.00

Refinement procedure

Resolution	32.900 - 2.100
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.900	2.130
High resolution limit [Å]	2.100	2.100
Number of reflections	58755	2518
<I/σ(I)>	4.8
Completeness [%]	100.0
Redundancy	573.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	Ammonium acetate, Sodium citrate tribasic dihydrate, PEG 4000, Benzamidine Hydrochloride