6PTL

Structure of the self-association domain of the chromatin looping factor LDB1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-09-27
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9537
Spacegroup name	P 32 2 1
Unit cell lengths	81.869, 81.869, 66.828
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.480 - 2.500
R-factor	0.2463
Rwork	0.245
R-free	0.27080
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.001
RMSD bond angle	1.140
Data reduction software	HKL-2000 (2.3.12)
Data scaling software	SCALEPACK
Phasing software	PHASER (2.8.2)
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.500	2.850	2.600
High resolution limit [Å]	2.500	2.800	2.500
Rmerge	0.083	1.474	1.213
Rmeas	0.087	2.256	1.271
Rpim	0.027	0.663	0.381
Number of reflections	9212	668	1015
<I/σ(I)>	14.7	1.4	2.5
Completeness [%]	99.7	100	100
Redundancy	10.7	11.6	11
CC(1/2)	0.998	0.600	0.929

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	18.6% PEG3350, 140 mM sodium phosphate dibasic
2	VAPOR DIFFUSION, SITTING DROP		293	18.6% PEG3350, 140 mM sodium phosphate dibasic, crystal soaked overnight in mother liquor containing 10 mM ethylmercury thiosalicylic acid